Research articles

2017

Harami, G. M., Seol, Y., In, J., Ferencziová, V., Martina, M., Gyimesi, M., Sarlós, K., Kovács, Z., J., Nagy, N. T., Sun, Y., Vellai, T., Neuman, K. C.*, Kovács, M.* (2017): Shuttling along DNA and directed processing of D-loops by RecQ helicase support quality control of homologous recombination. Proc Natl Acad Sci USA 114: E466-E475.
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2016

Gyimesi, M., Harami, G. M., Kocsis, Z. S., Kovács, M. (2016): Recent adaptations of fluorescence techniques for the determination of mechanistic parameters of helicases and translocases. Methods 108:24-39.
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Preface to Special Methods collection on DNA helicases



Gógl, G., Alexa, A., Kiss, B., Katona, G., Kovács, M., Reményi, A., Nyitray, L. (2016): Structural basis of Ribosomal S6 Kinase 1 (RSK1) inhibition by S100B Protein: modulation of the Extracellular Signal-regulated Kinase (ERK) signaling cascade in a calcium-dependent way. J. Biol. Chem. 291: 11-27.
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Paper of the Week announcement



2015

Harami, G. M., Nagy, N. T., Martina, M., Neuman, K. C., Kovács, M. (2015): The HRDC domain of E. coli RecQ helicase controls single-stranded DNA translocation and double-stranded DNA unwinding rates without affecting mechanoenzymatic coupling. Sci. Rep. 5: Article no. 11091.
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Sarlós, K., Gyimesi, M., Kele, Z., Kovács, M. (2015): Mechanism of RecQ helicase mechanoenzymatic coupling reveals that the DNA interactions of the ADP-bound enzyme control translocation run terminations. Nucleic Acids Res. 43: 1090-7.
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2014

Kocsis, Z.S.*, Sarlós, K.*, Harami, G.M., Martina, M, Kovács, M. (2014): A nucleotide-dependent and HRDC domain-dependent structural transition in DNA-bound RecQ helicase. J. Biol. Chem. 289: 5938-49.
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2013

Bibó, A., Kovács, M., Károlyi, G. (2013): Overdamped mechanical model of myosin II. Periodica Polytechnica Civil Engineering 57: 11-19.
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Nagy, N. T., Chakraborty,S., Harami, G.M., Sellers, J. R., Sakamoto, T., Kovács, M. (2013): A subdomain interaction at the base of the lever allosterically tunes the mechanochemical mechanism of myosin 5a. PLoS One 8: e62640.
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Harami, G.M., Gyimesi, M., Kovács, M. (2013): From keys to bulldozers: expanding roles for winged helix domains in nucleic-acid-binding proteins. Trends Biochem. Sci. 38: 364-71.
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Gyimesi, M., Pires, R. H., Billington, N., Sarlós, K., Kocsis, Z. S., Módos, K., Kellermayer, M. S., Kovács, M. (2013): Visualization of human Bloom’s syndrome helicase molecules bound to homologous recombination intermediates. FASEB J., 2013 Sep 4. [Epub ahead of print]
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2012

Ma, X.*, Kovács, M.*, Conti, M. A., Wang, A., Zhang, Y., Sellers, J. R., Adelstein, R. S. (2012): Nonmuscle myosin II exerts tension but does not translocate actin in vertebrate cytokinesis. Proc Natl Acad Sci U S A. 109: 4509-14.
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Gyimesi, M., Harami, G. M., Sarlós, K., Hazai, E., Bikádi, Z., Kovács, M. (2012): Complex activities of the human Bloom's syndrome helicase are encoded in a core region comprising the RecA and Zn-binding domains. Nucleic Acids Res. 40: 3952-63.
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Képiró, M., Várkuti, B. H., Bodor, A., Hegyi, G., Drahos, L., Kovács, M.*, Málnási-Csizmadia, A.* (2012): Azidoblebbistatin, a photoreactive myosin inhibitor. Proc Natl Acad Sci USA 109: 9402-7.
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Sarlós, K., Gyimesi, M., Kovács, M. (2012): RecQ helicase translocates along single-stranded DNA with a moderate processivity and tight mechanochemical coupling. Proc Natl Acad Sci USA 109: 9804-9.
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Biedermannova, L., Prokop, Z., Gora, A., Chovancova, E., Kovács, M., Damborsky, J., Wade, R. C. (2012): A single mutation in a tunnel to the active site changes the mechanism and kinetics of product release in haloalkane dehalogenase LinB. J. Biol. Chem. 2012 Jun 28. [Epub ahead of print]
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Ujfalusi, Z., Kovács, M., Nagy, N. T., Barko, S., Hild, G., Lukacs, A., Nyitrai, M., Bugyi, B. (2012): Myosin and tropomyosin stabilize the conformation of formin-nucleated actin filaments. J. Biol. Chem. 2012 Jun 29. [Epub ahead of print]
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2011

Takács, B., O'Neall-Hennessey, E., Hetényi, C., Kardos, J., Szent-Györgyi, A. G., Kovács, M. (2010): Myosin cleft closure determines the energetics of the actomyosin interaction. FASEB J. 25: 111-121.
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2010

Bibó, A. Kovács, M., Károlyi, G. (2010): Internal lever arm model for myosin II. In: G. Stépán, L. L. Kovács, A. Tóth (Ed.) IUTAM Symposium on Dynamics Modeling and Interaction Control in Virtual and Real Environments. Springer, pp. 155-163. IUTAM Bookseries; 30. ISBN: 978-94-007-1642-1
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Radnai, L., Rapali, P., Hodi, Z., Suveges, D., Molnar, T., Kiss, B., Becsi, B., Erdodi, F., Buday, L., Kardos, J., Kovacs, M., Nyitray L. (2010): Affinity, avidity and kinetics of target sequence binding to LC8 dynein light chain isoforms. J. Biol. Chem. 285: 38649-38657.
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Málnási-Csizmadia, A., Kovács, M. (2010): Emerging complex pathways of the actomyosin powerstroke. Trends Biochem Sci. 35: 684-690.
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Nagy, N. T., Sakamoto, T., Takács, B., Gyimesi, M., Hazai, E., Bikádi, Z., Sellers, J. R., Kovács, M. (2010): Functional adaptation of the switch-2 nucleotide sensor enables rapid processive translocation by myosin-5. FASEB J. 24: 4480-4490.
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Takács, B., Billington, N., Gyimesi, M., Kintses, B., Málnási-Csizmadia, A., Knight, P. J., Kovács, M. (2010): Myosin complexed with ADP and blebbistatin reversibly adopts a conformation resembling the start point of the working stroke. Proc Natl Acad Sci U S A. 107: 6799-804.
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Gyimesi, M., Sarlós, K., Kovács, M. (2010): Processive translocation mechanism of the human Bloom's syndrome helicase along single-stranded DNA. Nucleic Acids Research 38: 4404-14.
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Gyimesi, M., Sarlós, K., Derényi, I., Kovács, M. (2010): Streamlined determination of processive run length and mechanochemical coupling of nucleic acid motor activities. Nucleic Acids Research 38: e102.

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2009

Forgacs, E., Sakamoto, T., Cartwright, S., Belknap, B., Kovács, M., Tóth, J., Webb, M. R., Sellers, J. R., White, H. D. (2009): Switch 1 Mutation S217A Converts Myosin V into a Low Duty Ratio Motor. J. Biol. Chem. 284: 2138-49.
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2008

Takagi, Y., Yang, Y., Fujiwara, I., Jacobs, D., Cheney, R. E., Sellers, J. R., Kovács, M. (2008): Human myosin Vc is a low duty ratio, non-processive molecular motor. J. Biol. Chem. 283: 8527-37
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2007

Iwamoto, H., Oiwa, K., Kovács, M., Sellers, J. R., Suzuki, T., Wakayama, J., Tamura, T., Yagi, N., Fujisawa, T. (2007):
Diversity of structural behavior in vertebrate conventional myosins complexed with actin. J. Mol. Biol. 369: 249-64.
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Málnási-Csizmadia, A., Tóth, J., Pearson, D. S., Hetényi, C., Nyitray, L., Geeves, M. A., Bagshaw, C. R., Kovács, M. (2007):
Selective perturbation of the myosin recovery stroke by point mutations at the base of the lever arm affects ATP hydrolysis and phosphate release. J. Biol. Chem. 282: 17658-64.
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Yang, Y., Gourinath, S., Kovács, M., Nyitray, L., Reutzel, R., Himmel, D. M., O'Neall-Hennessey, E., Reshetnikova, L., Szent-Györgyi, A. G., Brown, J. H., Cohen, C. (2007):
Rigor-like structures of muscle myosins reveal key mechanical elements in the transduction pathways of this allosteric motor. Structure 15: 553-64.
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Kovács, M., Thirumurugan, K., Knight, P. J., Sellers, J. R. (2007):
Load-dependent mechanism of non-muscle myosin 2. Proc. Natl. Acad. Sci. USA. 104: 9994-9.
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Tóth, J., Varga, B., Kovács, M., Málnási-Csizmadia, A., Vértessy, B. G. (2007):
Kinetic mechanism of human dUTPase, an essential nucleotide pyrophosphatase enzyme. J. Biol. Chem. 282: 33572-82.
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2006

Yang, Y.*, Kovács, M.*, Sakamoto, T.*, Zhang, F., Kiehart, D. P., Sellers, J. R. (2006):
Dimerized Drosophila myosin VIIa: a processive motor. Proc. Natl. Acad. Sci. USA 103: 5746-51.
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Forgács, E., Cartwright, S., Kovács, M., Sakamoto, T., Sellers, J. R., Corrie, J. E., Webb, M. R., White, H. D. (2006):
Kinetic mechanism of myosinV-S1 using a new fluorescent ATP analogue. Biochemistry 45:13035-45.
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Kintses, B., Simon, Z., Gyimesi, M., Tóth, J., Jelinek, B., Niedetzky, C., Kovács, M., Málnási-Csizmadia, A. (2006):
Enzyme kinetics above denaturation temperature: a temperature-jump/stopped-flow apparatus. Biophys J.91: 4605-10.
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2005

Kovács, M., Wang, F., Sellers, J. R. (2005):
Mechanism of action of myosin X, a membrane-associated molecular motor. J. Biol. Chem. 280: 15071-15083.
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Kim, K-Y.*, Kovács, M.*, Kawamoto, S., Sellers, J. R., Adelstein, R. S. (2005):
Disease-associated mutations and alternative splicing alter the enzymatic and motile activity of nonmuscle myosins II-B and II-C. J. Biol. Chem. 280: 22769-75.
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Liu, X., Shu, S., Kovács, M., Korn, E. D. (2005):
Biological, biochemical and kinetic effects of mutations of the cardiomyopathy-loop of Dictyostelium myosin II: Importance of Ala400. J. Biol. Chem.280: 26974-83.
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Tóth, J., Kovács, M., Wang, F., Nyitray, L., Sellers, J. R. (2005):
Myosin V from Drosophila reveals diversity of motor mechanisms within the myosin V family. J. Biol. Chem. 280: 30594-603.
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Yang, Y.*, Kovács, M.*, Xu, E., Anderson, J. B., Sellers, J. R. (2005):
Myosin VIIB from Drosophila is a high duty ratio motor. J. Biol. Chem. 280: 32061-8.
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2004

Kovács, M., Tóth, J., Nyitray, L., Sellers, J. R. (2004):
Two-headed binding of the unphosphorylated non-muscle heavy meromyosin-ADP complex to actin. Biochemistry 43: 4219-26.
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Kovács, M., Tóth, J., Málnási-Csizmadia, A., Bagshaw, C. R., Nyitray, L. (2004):
Engineering lysine reactivity as a conformational sensor in the Dictyostelium myosin II motor domain. J. Muscle Res. Cell Motil. 25: 95-102.
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Kovács, M., Tóth, J., Hetényi, C., Málnási-Csizmadia, A., Sellers, J. R. (2004):
Mechanism of blebbistatin inhibition of myosin II. J. Biol. Chem. 279: 35557-63.
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2003

Wang, F.*, Kovács, M.*, Hu, A., Limouze, J., Harvey, E. V., Sellers, J. R. (2003):
Kinetic mechanism of non-muscle myosin IIB: functional adaptations for tension generation and maintenance. J. Biol. Chem. 278: 27439-48.
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Kovács, M.*, Wang, F.*, Hu, A., Zhang, Y., Sellers, J. R. (2003):
Functional divergence of human cytoplasmic myosin II: kinetic characterization of the non-muscle IIA isoform. J. Biol. Chem. 278: 38132-40.
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Conibear, P. B., Bagshaw, C. R., Fajer, P., Kovács, M., Málnási-Csizmadia, A. (2003):
Myosin cleft movement and its coupling to actomyosin dissociation. Nat. Struct. Biol. 10: 831-5.
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2002

Kovács, M., Málnási-Csizmadia, A., Woolley, J. R., Bagshaw, C. R. (2002):
Analysis of nucleotide binding to Dictyostelium myosin II motor domains containing a single tryptophan near the active site. J. Biol. Chem. 277: 28459-67.
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Wakelin, S., Woolley, R. J., Floyd, D. N., Bagshaw, C. R., Kovács, M., Málnási-Csizmadia, A. (2002):
Engineering Dictyostelium discoideum myosin II for the introduction of site-specific fluorescence probes. J. Muscle Res. Cell Motil. 23: 673-683.
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2001

Málnási-Csizmadia, A., Kovács, M., Woolley, J. R., Botchway, S. W., Bagshaw, C. R. (2001):
The dynamics of the relay loop tryptophan residue in the Dictyostelium myosin motor domain and the origin of spectroscopic signals. J. Biol. Chem. 276: 19483-90.
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Málnási-Csizmadia, A., Pearson, D. S., Kovács, M., Woolley, R. J., Geeves, M. A., Bagshaw, C. R. (2001):
Kinetic resolution of a conformational transition and the ATP hydrolysis step using relaxation methods with a Dictyostelium myosin II mutant containing a single tryptophan residue. Biochemistry 40: 12727-37.
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